1. RODNA
  2. FCEN
  3. FCEN - Facultad de Ciencias Exactas y Naturales. UBA
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.provenanceFacultad de Ciencias Exactas y Naturales de la UBA-
dc.contributor<div class="autor_fcen" id="3622">Genovese, G.</div>-
dc.contributorSenek, M.-
dc.contributor<div class="autor_fcen" id="6291">Ortiz, N.</div>-
dc.contributor<div class="autor_fcen" id="7164">Regueira, M.</div>-
dc.contributorTowle, D.W.-
dc.contributor<div class="autor_fcen" id="8656">Tresguerres, M.</div>-
dc.contributor<div class="autor_fcen" id="5211">Luquet, C.M.</div>-
dc.creator<div class="autor_fcen" id="3622">Genovese, G.</div>-
dc.creatorSenek, M.-
dc.creator<div class="autor_fcen" id="6291">Ortiz, N.</div>-
dc.creator<div class="autor_fcen" id="7164">Regueira, M.</div>-
dc.creatorTowle, D.W.-
dc.creator<div class="autor_fcen" id="8656">Tresguerres, M.</div>-
dc.creator<div class="autor_fcen" id="5211">Luquet, C.M.</div>-
dc.date.accessioned2018-05-04T22:04:55Z-
dc.date.accessioned2018-05-28T15:47:57Z-
dc.date.available2018-05-04T22:04:55Z-
dc.date.available2018-05-28T15:47:57Z-
dc.date.issued2006-
dc.identifier.urihttp://10.0.0.11:8080/jspui/handle/bnmm/68388-
dc.descriptionThe effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial potential difference (V tc) from 2.2±0.2 to 4.8±0.3 mV, describing an initial cAMP-dependent stimulating phase followed by an inhibitory phase. Spiperone and domperidone (antagonists of D2-like DA receptors in vertebrates) completely blocked the response to DA, while the D1-like antagonist SCH23390 blocked only the inhibitory phase. Theophylline (phosphodiesterase inhibitor) and okadaic acid (protein phosphatases PP1 and PP2A inhibitor) were also able to block the inhibitory phase, suggesting that it depends on adenylyl cyclase inhibition and on protein phosphatases. When the gills were perfused with hypoosmotic solution, or with the adenylyl cyclase activator forskolin, V te was increased several-fold. DA applied under these stimulated conditions partially reversed the Vte increase by 54% and 25%, respectively. Similarly, the D1-like agonist, fenoldopam, produced a 33% reduction in the stimulated V te. We propose that, in C. granulatus gills, DA stimulates adenylyl cyclase and therefore ion transport through D1-like receptors linked to a Gs protein, although they respond to antagonists that interact with D2-like receptors in vertebrates. The inhibitory phase seems to be mediated by D2-like receptors linked to a Gi/o protein, which inhibits adenylyl cyclase, although these receptors can be activated or blocked by agonists or antagonists that interact with D1-like receptors in vertebrates and insects.-
dc.descriptionFil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Ortiz, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Regueira, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Tresguerres, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.descriptionFil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by/2.5/ar-
dc.sourceJ. Exp. Biol. 2006;209(14):2785-2793-
dc.source.urihttp://digital.bl.fcen.uba.ar/Download/paper/paper_00220949_v209_n14_p2785_Genovese.pdf-
dc.subjectcAMP-
dc.subjectChasmagnathus granulatus-
dc.subjectCrab-
dc.subjectDopamine antagonist-
dc.subjectDopamine receptor dopamine agonist-
dc.subjectNa +/K +-ATPase-
dc.subjectOkadaic acid-
dc.subjectTransepithelial potential differences-
dc.subjectadenosine triphosphatase (potassium sodium)-
dc.subjectcyclic AMP dependent protein kinase-
dc.subjectdopamine 1 receptor-
dc.subjectdopamine 2 receptor-
dc.subjectokadaic acid-
dc.subjecttheophylline-
dc.subjectanimal-
dc.subjectarticle-
dc.subjectBrachyura-
dc.subjectgill-
dc.subjection transport-
dc.subjectmale-
dc.subjectmetabolism-
dc.subjectosmolarity-
dc.subjectphysiology-
dc.subjectsignal transduction-
dc.subjectAnimals-
dc.subjectBrachyura-
dc.subjectCyclic AMP-Dependent Protein Kinases-
dc.subjectGills-
dc.subjectIon Transport-
dc.subjectMale-
dc.subjectNa(+)-K(+)-Exchanging ATPase-
dc.subjectOkadaic Acid-
dc.subjectOsmolar Concentration-
dc.subjectReceptors, Dopamine D1-
dc.subjectReceptors, Dopamine D2-
dc.subjectSignal Transduction-
dc.subjectTheophylline-
dc.subjectChasmagnathus granulata-
dc.subjectDecapoda (Crustacea)-
dc.subjectHexapoda-
dc.subjectInsecta-
dc.subjectVertebrata-
dc.titleDopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:ar-repo/semantics/artículo-
dc.typeinfo:eu-repo/semantics/publishedVersion-
Aparece en las colecciones: FCEN - Facultad de Ciencias Exactas y Naturales. UBA

Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro sencillo del ítem