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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Universidad de Buenos Aires. Facultad de Ciencias Económicas | - |
| dc.creator | Liu, Li | - |
| dc.creator | Ikeda, Tatsuya M. | - |
| dc.creator | Branlard, Gerard | - |
| dc.creator | Peña, Roberto J. | - |
| dc.creator | Rogers, John William | - |
| dc.creator | Lerner, Silvia E. | - |
| dc.creator | Kolman, Maía de Los Angeles | - |
| dc.creator | Xia, Xianchun | - |
| dc.creator | Wang, Linhai | - |
| dc.creator | Ma, Wujun | - |
| dc.creator | Appels, Rudi | - |
| dc.creator | Yoshida, Hisashi | - |
| dc.creator | Wang, Aili | - |
| dc.creator | Yan, Yueming | - |
| dc.creator | He, Zhonghu | - |
| dc.date | 2010 | - |
| dc.date.accessioned | 2019-06-19T20:34:52Z | - |
| dc.date.available | 2019-06-19T20:34:52Z | - |
| dc.date.issued | 2010 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/7137 | - |
| dc.identifier | Recurso Completo | - |
| dc.identifier.uri | http://rodna.bn.gov.ar/jspui/handle/bnmm/326812 | - |
| dc.description | <strong>Background</strong> Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. <strong>Results</strong> At the<em>Glu-A3</em>locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles<em>Glu-A3e</em>and<em>Glu-A3d</em>could not be routinely distinguished from<em>Glu-A3f</em>and<em>Glu-A3g</em>, respectively, based on SDS-PAGE, and the allele<em>Glu-A3a</em>could not be differentiated from<em>Glu-A3c</em>by MALDI-TOF-MS. At the<em>Glu-B3</em>locus, alleles<em>Glu-B3a</em>,<em>Glu-B3b</em>,<em>Glu-B3c</em>,<em>Glu-B3g</em>,<em>Glu-B3h</em>and<em>Glu-B3j</em>could be clearly identified by all four methods, whereas<em>Glu-B3ab</em>,<em>Glu-B3ac</em>,<em>Glu-B3ad</em>could only be identified by the 2-DE method. At the<em>Glu-D3</em>locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the<em>Glu-D3</em>alleles. <strong>Conclusions</strong> PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of<em>Glu-A3</em>and<em>Glu-B3</em>alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels.<em>Glu-D3c</em>and<em>Glu-D3e</em>are the same allele. Two new alleles, namely,<em>Glu-D3m</em>in cultivar Darius, and<em>Glu-D3n</em>in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat. | - |
| dc.format | application/pdf | - |
| dc.format | 24 p. | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution 4.0 International (BY 4.0) | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/7137 | - |
| dc.source.uri | Recurso Completo | - |
| dc.subject | Agronomía, reproducción y protección de plantas | - |
| dc.title | Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/submittedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Facultad de Ciencias Económicas. UBA | |
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