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dc.provenanceINTA-
dc.contributorGimenez, Magali Diana-
dc.contributorYañez Santos, Anahi Mara-
dc.contributorPaz, Rosalia Cristina-
dc.contributorQuiroga, Mariana Paola-
dc.contributorMarfil, Carlos Fedrico-
dc.contributorConci, Vilma Cecilia-
dc.contributorGarcia Lampasona, Sandra Claudia-
dc.creatorGimenez, Magali Diana-
dc.creatorYañez Santos, Anahi Mara-
dc.creatorPaz, Rosalia Cristina-
dc.creatorQuiroga, Mariana Paola-
dc.creatorMarfil, Carlos Fedrico-
dc.creatorConci, Vilma Cecilia-
dc.creatorGarcia Lampasona, Sandra Claudia-
dc.date2017-11-01T12:16:47Z-
dc.date2017-11-01T12:16:47Z-
dc.date2016-01-
dc.date.accessioned2019-04-29T16:28:06Z-
dc.date.available2019-04-29T16:28:06Z-
dc.date.issued2017-11-01T12:16:47Z-
dc.date.issued2017-11-01T12:16:47Z-
dc.date.issued2016-01-
dc.identifier0721-7714 (Print)-
dc.identifier1432-203X (Online)-
dc.identifierhttps://doi.org/10.1007/s00299-015-1874-x-
dc.identifierhttp://hdl.handle.net/20.500.12123/1640-
dc.identifierhttps://link.springer.com/article/10.1007%2Fs00299-015-1874-x-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313823-
dc.descriptionGarlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic.-
dc.descriptionEEA Mendoza-
dc.descriptionFil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina-
dc.descriptionFil: Yañez Santos, Anahi Mara. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina-
dc.descriptionFil: Paz, Rosalia Cristina. Universidad Nacional de San Juan-Consejo Nacional de Investigaciones Científicas y Técnicas. CIGEOBIO; Argentina-
dc.descriptionFil: Marfil, Carlos Fedrico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina-
dc.descriptionFil: Conci, Vilma Cecilia. INTA. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Garcia Lampasona, Sandra Claudia. INTA. Estación Experimental Agropecuaria Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina-
dc.descriptionFil: Quiroga, Mariana Paola. INTA. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourcePlant Cell Reports 35 (1) : 129–141 (January 2016)-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.uri0721-7714 (Print)-
dc.source.uri1432-203X (Online)-
dc.source.urihttps://doi.org/10.1007/s00299-015-1874-x-
dc.source.urihttp://hdl.handle.net/20.500.12123/1640-
dc.source.urihttps://link.springer.com/article/10.1007%2Fs00299-015-1874-x-
dc.source.urihttps://doi.org/10.1007/s00299-015-1874-x-
dc.source.urihttp://hdl.handle.net/20.500.12123/1640-
dc.source.urihttps://link.springer.com/article/10.1007%2Fs00299-015-1874-x-
dc.subjectGenética-
dc.subjectAjo-
dc.subjectVariación Somaclonal-
dc.subjectExperimentación In Vitro-
dc.subjectAllium Sativum-
dc.subjectCultivo de Meristemas-
dc.subjectGenetics-
dc.subjectGarlic-
dc.subjectSomaclonal Variation-
dc.subjectIn Vitro Experimentation-
dc.subjectMeristem Culture-
dc.titleAssessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro propagation-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/acceptedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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