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dc.provenanceINTA-
dc.contributorHelguera, Marcelo-
dc.contributorRivarola, Maximo Lisandro-
dc.contributorClavijo, Bernardo-
dc.contributorMartis, Mihaela M.-
dc.contributorVanzetti, Leonardo Sebastian-
dc.contributorGonzalez, Sergio Alberto-
dc.contributorGarbus, Ingrid-
dc.contributorLeroy, Phillippe-
dc.contributorSimková, Hana-
dc.contributorValárik, Miroslav-
dc.contributorCaccamo, Mario-
dc.contributorDolezel, Jaroslav-
dc.contributorMayer, Klaus F.X.-
dc.contributorFeuillet, Catherine-
dc.contributorTranquilli, Gabriela-
dc.contributorPaniego, Norma Beatriz-
dc.contributorEchenique, Carmen Viviana-
dc.creatorHelguera, Marcelo-
dc.creatorRivarola, Maximo Lisandro-
dc.creatorClavijo, Bernardo-
dc.creatorMartis, Mihaela M.-
dc.creatorVanzetti, Leonardo Sebastian-
dc.creatorGonzalez, Sergio Alberto-
dc.creatorGarbus, Ingrid-
dc.creatorLeroy, Phillippe-
dc.creatorSimková, Hana-
dc.creatorValárik, Miroslav-
dc.creatorCaccamo, Mario-
dc.creatorDolezel, Jaroslav-
dc.creatorMayer, Klaus F.X.-
dc.creatorFeuillet, Catherine-
dc.creatorTranquilli, Gabriela-
dc.creatorPaniego, Norma Beatriz-
dc.creatorEchenique, Carmen Viviana-
dc.date2017-09-06T12:48:36Z-
dc.date2017-09-06T12:48:36Z-
dc.date2015-
dc.date.accessioned2019-04-29T16:26:50Z-
dc.date.available2019-04-29T16:26:50Z-
dc.date.issued2017-09-06T12:48:36Z-
dc.date.issued2017-09-06T12:48:36Z-
dc.date.issued2015-
dc.identifier0168-9452-
dc.identifierhttps://doi.org/10.1016/j.plantsci.2014.12.004-
dc.identifierhttp://hdl.handle.net/20.500.12123/1142-
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352925/-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313418-
dc.descriptionSurvey sequencing of the bread wheat (Triticum aestivum L.) genome (AABBDD) has been approached through different strategies delivering important information. However, the current wheat sequence knowledge is not complete. The aim of our study is to provide different and complementary set of data for chromosome 4D. A survey sequence was obtained by pyrosequencing of flow-sorted 4DS (7.2×) and 4DL (4.1×) arms. Single ends (SE) and long mate pairs (LMP) reads were assembled into contigs (223 Mb) and scaffolds (65 Mb) that were aligned to Aegilops tauschii draft genome (DD), anchoring 34 Mb to chromosome 4. Scaffolds annotation rendered 822 gene models. A virtual gene order comprising 1973 wheat orthologous gene loci and 381 wheat gene models was built. This order was largely consistent with the scaffold order determined based on a published high density map from the Ae. tauschii chromosome 4, using bin-mapped 4D ESTs as a common reference. The virtual order showed a higher collinearity with homeologous 4B compared to 4A. Additionally, a virtual map was constructed and ∼5700 genes (∼2200 on 4DS and ∼3500 on 4DL) predicted. The sequence and virtual order obtained here using the 454 platform were compared with the Illumina one used by the IWGSC, giving complementary information-
dc.descriptionFil: Helguera, Marcelo. INTA. Estación Experimental Agropecuaria Marcos Juárez; Argentina-
dc.descriptionFil: Rivarola, Maximo Lisandro. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Clavijo, Bernardo. Norwich Research Park. The Genome Analysis Centre; Reino Unido-
dc.descriptionFil: Martis, Mihaela M.. Helmholtz Zentrum München; Alemania-
dc.descriptionFil: Vanzetti, Leonardo Sebastián. INTA. Estación Experimental Agropecuario Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Gonzalez, Sergio Alberto. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur; Argentina-
dc.descriptionFil: Leroy, Phillippe. Universite Blaise Pascal; Francia. Institut National de la Recherche Agronomique; Francia-
dc.descriptionFil: Šimková, Hana. Institute of Experimental Botany. Centre of the Region Haná for Biotechnological and Agricultural Research; República Checa-
dc.descriptionFil: Valárik, Miroslav. Institute of Experimental Botany. Centre of the Region Haná for Biotechnological and Agricultural Research; República Checa-
dc.descriptionFil: Caccamo, Mario. Norwich Research Park. The Genome Analysis Centre; Reino Unido-
dc.descriptionFil: Doležel, Jeroslav. Institute of Experimental Botany. Centre of the Region Haná for Biotechnological and Agricultural Research; República Checa-
dc.descriptionFil: Mayer, Klaus F. X.. Helmholtz Zentrum München; Alemania-
dc.descriptionFil: Feuillet, Catherine. Bayer Crop Science; Estados Unidos-
dc.descriptionFil: Tranquilli, Gabriela. INTA. Instituto de Recursos Biológicos; Argentina-
dc.descriptionFil: Paniego, Norma Beatriz. INTA. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur; Argentina-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourcePlant science 233 : 200-212. (Apr. 2015)-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.uri0168-9452-
dc.source.urihttps://doi.org/10.1016/j.plantsci.2014.12.004-
dc.source.urihttp://hdl.handle.net/20.500.12123/1142-
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352925/-
dc.source.urihttps://doi.org/10.1016/j.plantsci.2014.12.004-
dc.source.urihttp://hdl.handle.net/20.500.12123/1142-
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352925/-
dc.subjectTrigo-
dc.subjectTriticum Aestivum-
dc.subjectGenes-
dc.subjectCromosomas-
dc.subjectChromosomes-
dc.subjectCromosoma 4 D-
dc.titleNew insight into the wheat chromosome 4 D structure and virtual gene order, relead by survey pyrosequencing-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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