Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | INTA | - |
| dc.contributor | Rabinovitz, Bettina Carol | - |
| dc.contributor | Larzábal, M. | - |
| dc.contributor | Vilte, Daniel Alejandro | - |
| dc.contributor | Cataldi, Angel Adrian | - |
| dc.contributor | Mercado, Elsa Cristina | - |
| dc.creator | Rabinovitz, Bettina Carol | - |
| dc.creator | Larzábal, M. | - |
| dc.creator | Vilte, Daniel Alejandro | - |
| dc.creator | Cataldi, Angel Adrian | - |
| dc.creator | Mercado, Elsa Cristina | - |
| dc.date | 2017-08-22T13:22:37Z | - |
| dc.date | 2017-08-22T13:22:37Z | - |
| dc.date | 2016-05 | - |
| dc.date.accessioned | 2019-04-29T16:26:29Z | - |
| dc.date.available | 2019-04-29T16:26:29Z | - |
| dc.date.issued | 2017-08-22T13:22:37Z | - |
| dc.date.issued | 2017-08-22T13:22:37Z | - |
| dc.date.issued | 2016-05 | - |
| dc.identifier | 0264-410X | - |
| dc.identifier | https://doi.org/10.1016/j.vaccine.2016.04.056 | - |
| dc.identifier | http://hdl.handle.net/20.500.12123/1001 | - |
| dc.identifier | http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313299 | - |
| dc.description | Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.source | Vaccine 34 (25) : 2793-2797 (May 2016) | - |
| dc.source | reponame:INTA Digital (INTA) | - |
| dc.source | instname:Instituto Nacional de Tecnología Agropecuaria | - |
| dc.source | instacron:INTA | - |
| dc.source.uri | 0264-410X | - |
| dc.source.uri | https://doi.org/10.1016/j.vaccine.2016.04.056 | - |
| dc.source.uri | http://hdl.handle.net/20.500.12123/1001 | - |
| dc.source.uri | http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 | - |
| dc.source.uri | https://doi.org/10.1016/j.vaccine.2016.04.056 | - |
| dc.source.uri | http://hdl.handle.net/20.500.12123/1001 | - |
| dc.source.uri | http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 | - |
| dc.subject | Escherichia coli | - |
| dc.subject | Vaccination | - |
| dc.subject | Mice | - |
| dc.subject | Infection | - |
| dc.subject | Vacunación | - |
| dc.subject | Ratón | - |
| dc.subject | Infección | - |
| dc.title | The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/acceptedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | INTA | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.