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dc.provenanceINTA-
dc.contributorBenintende, Graciela B.-
dc.contributorBerretta, Marcelo Facundo-
dc.contributorZandomeni, Rubén O.-
dc.contributorSauka, Diego H.-
dc.contributorAmadio, Ariel F.-
dc.contributorOrtiz, Elio M.-
dc.contributorNavas, Laura E.-
dc.creatorBenintende, Graciela B.-
dc.creatorBerretta, Marcelo Facundo-
dc.creatorZandomeni, Rubén O.-
dc.creatorSauka, Diego H.-
dc.creatorAmadio, Ariel F.-
dc.creatorOrtiz, Elio M.-
dc.creatorNavas, Laura E.-
dc.date2017-07-10T18:07:44Z-
dc.date2017-07-10T18:07:44Z-
dc.date2017-
dc.date.accessioned2019-04-29T16:25:38Z-
dc.date.available2019-04-29T16:25:38Z-
dc.date.issued2017-07-10T18:07:44Z-
dc.date.issued2017-07-10T18:07:44Z-
dc.date.issued2017-
dc.identifierhttps://doi.org/10.1159/000451056-
dc.identifier1660-2412-
dc.identifierhttps://doi.org/10.1159/000451056-
dc.identifierhttp://hdl.handle.net/20.500.12123/629-
dc.identifierhttps://www.karger.com/Article/Abstract/451056-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/313022-
dc.descriptionWe report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.-
dc.descriptionEEA Rafaela-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.sourceJournal of Molecular Microbiology and Biotechnology 27 : 43-54-
dc.sourcereponame:INTA Digital (INTA)-
dc.sourceinstname:Instituto Nacional de Tecnología Agropecuaria-
dc.sourceinstacron:INTA-
dc.source.urihttps://doi.org/10.1159/000451056-
dc.source.uri1660-2412-
dc.source.urihttps://doi.org/10.1159/000451056-
dc.source.urihttp://hdl.handle.net/20.500.12123/629-
dc.source.urihttps://www.karger.com/Article/Abstract/451056-
dc.source.urihttps://doi.org/10.1159/000451056-
dc.source.urihttps://doi.org/10.1159/000451056-
dc.source.urihttp://hdl.handle.net/20.500.12123/629-
dc.source.urihttps://www.karger.com/Article/Abstract/451056-
dc.subjectGenética-
dc.subjectBacillus Thuringiensis-
dc.subjectGenes-
dc.subjectGenetics-
dc.subjectpFR260-
dc.subjectGenes Insecticidas-
dc.titleComplete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: INTA

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