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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Comisión de Investigaciones Científicas | - |
| dc.contributor | Consolo, Verónica Fabiana | - |
| dc.contributor | Albani, C.M. | - |
| dc.contributor | Berón, C.M. | - |
| dc.contributor | Salerno, Graciela Lidia | - |
| dc.contributor | Cordo, Cristina Alicia | - |
| dc.creator | Consolo, Verónica Fabiana | - |
| dc.creator | Albani, C.M. | - |
| dc.creator | Berón, C.M. | - |
| dc.creator | Salerno, Graciela Lidia | - |
| dc.creator | Cordo, Cristina Alicia | - |
| dc.date | 2009-05 | - |
| dc.date.accessioned | 2019-04-29T16:14:30Z | - |
| dc.date.available | 2019-04-29T16:14:30Z | - |
| dc.date.issued | 2009-05 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/6993 | - |
| dc.identifier | Recurso Completo | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/312337 | - |
| dc.description | A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. | - |
| dc.format | application/pdf | - |
| dc.format | 6 p. | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/6993 | - |
| dc.source.uri | Recurso Completo | - |
| dc.subject | Agronomía, reproducción y protección de plantas | - |
| dc.title | A conventional PCR technique to detect Septoria tritici in wheat seeds | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/submittedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Comisión de Investigaciones Científicas de la Prov. de Buenos Aires | |
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