1. RODNA
  2. CIC Digital
  3. Comisión de Investigaciones Científicas de la Prov. de Buenos Aires
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dc.provenanceComisión de Investigaciones Científicas-
dc.contributorIsla Larrain, Marina Teresita-
dc.contributorDemichelis, Sandra O.-
dc.contributorCrespo, Marina-
dc.contributorLacunza, Ezequiel-
dc.contributorBarbera, Alberto-
dc.contributorCreton, Aldo-
dc.contributorTerrier, Francisco-
dc.contributorSegal-Eiras, Amada-
dc.contributorCroce, María Virginia-
dc.creatorIsla Larrain, Marina Teresita-
dc.creatorDemichelis, Sandra O.-
dc.creatorCrespo, Marina-
dc.creatorLacunza, Ezequiel-
dc.creatorBarbera, Alberto-
dc.creatorCreton, Aldo-
dc.creatorTerrier, Francisco-
dc.creatorSegal-Eiras, Amada-
dc.creatorCroce, María Virginia-
dc.date2009-01-01-
dc.date.accessioned2019-04-29T16:08:40Z-
dc.date.available2019-04-29T16:08:40Z-
dc.date.issued2009-01-01-
dc.identifierhttp://digital.cic.gba.gob.ar/handle/11746/3754-
dc.identifierRegistro completo-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/310240-
dc.descriptionIn cancer patients, MUC1 glycoprotein may carry Lewis y which could be involved in immune response. Purposes: 1- to evaluate the presence of Lewis y and MUC1 in circulating immune complexes (Lewis y/CIC and MUC1/CIC, respectively) and their correlation; 2- to analyze the possible presence of Lewis y in carbohydrate chains of tumoral MUC1 glycoprotein and 3- to correlate serum and tissue parameters considered. Pretreatment serum and tissue breast samples from 76 adenocarcinoma, 34 benign and 36 normal specimens were analyzed. Anti-MUC1 and anti-Lewis y MAbs were employed. To detect Lewis y/CIC and MUC1/CIC, ELISA tests were developed; serum samples containing MUC1 were previously selected by Cancer Associated Serum Antigen (CASA). Immunoprecipitation (IP) was performed in 9 malignant, benign and normal samples and analyzed by SDS-PAGE and Western blot. Lewis y and MUC1 expression was studied by immunohistochemistry (IHC). Statistical analysis was performed employing principal component analysis (PCA), ANOVA, Tukey HSD, Chi square test and classical correlation (p < 0.05). By ELISA, Lewis y/IgM/CIC levels showed statistically significant differences between breast cancer versus benign and normal samples; mean +/- SD values expressed in OD units were: 0.525 +/- 0.304; 0.968 +/- 0.482 and 0.928 +/- 0.447, for breast cancer, benign disease and normal samples, respectively, p < 0.05. Lewis y/IgG/CIC did not show any statistically significant difference. MUC1/IgM/CIC correlated with Lewis y/IgM/CIC. By CASA, 9 samples with MUC1 values above the cut off were selected and IP was performed, followed by SDS-PAGE and Western blot; bands at 200 kDa were obtained with each MAb in all the samples. By IHC, with C14 MAb, 47.5%, 31% and 35% of malignant, benign and normal samples, respectively, showed positive reaction while all the samples were positive with anti-MUC1 MAb; in both cases, with a different pattern of expression between malignant and non malignant samples. Our findings support that in breast cancer there was a limited humoral immune response through Lewis y/IgM/CIC levels detection which correlated with MUC1/IgM/CIC. We also found that Lewis y might be part of circulating MUC1 glycoform structure and also that Lewis y/CIC did not correlate with Lewis y expression.-
dc.formatapplication/pdf-
dc.format11 p.-
dc.languageeng-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightsAttribution 4.0 International (BY 4.0)-
dc.sourcereponame:CIC Digital (CICBA)-
dc.sourceinstname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires-
dc.sourceinstacron:CICBA-
dc.source.urihttp://digital.cic.gba.gob.ar/handle/11746/3754-
dc.source.uriRegistro completo-
dc.subjectCiencias Médicas y de la Salud-
dc.titleBreast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/submittedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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