Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | Comisión de Investigaciones Científicas | - |
| dc.contributor | Muñoz, Fernando F. | - |
| dc.contributor | Mendieta, Julieta Renée | - |
| dc.contributor | Pagano, Mariana R. | - |
| dc.contributor | Paggi, Roberto A. | - |
| dc.contributor | Daleo, Gustavo Raúl | - |
| dc.contributor | Guevara, María G. | - |
| dc.creator | Muñoz, Fernando F. | - |
| dc.creator | Mendieta, Julieta Renée | - |
| dc.creator | Pagano, Mariana R. | - |
| dc.creator | Paggi, Roberto A. | - |
| dc.creator | Daleo, Gustavo Raúl | - |
| dc.creator | Guevara, María G. | - |
| dc.date | 2010-02-03 | - |
| dc.date.accessioned | 2019-04-29T16:07:57Z | - |
| dc.date.available | 2019-04-29T16:07:57Z | - |
| dc.date.issued | 2010-02-03 | - |
| dc.identifier | http://digital.cic.gba.gob.ar/handle/11746/5367 | - |
| dc.identifier | Recurso Completo | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/309977 | - |
| dc.description | Plant-specific insert domain (PSI) is a region of approximately 100 amino acid residues present in most plant aspartic protease (AP) precursors. PSI is not a true saposin domain; it is the exchange of the N- and C-terminal portions of the saposin like domain. Hence, PSI is called a swaposin domain. Here, we report the cloned, heterologous expression and purification of PSI from StAsp 1 (Solanum tuberosum aspartic protease 1), called StAsp-PSI. Results obtained here show that StAsp-PSI is able to kill spores of two potato pathogens in a dose-dependent manner without any deleterious effect on plant cells. As reported for StAPs (S. tuberosum aspartic proteases), the StAsp-PSI ability to kill microbial pathogens is dependent on the direct interaction of the protein with the microbial cell wall/or membrane, leading to increased permeability and lysis. Additionally, we demonstrated that, like proteins of the SAPLIP family, StAsp-PSI and StAPs are cytotoxic to Gram-negative and Gram-positive bacteria in a dose dependent manner. The amino acid residues conserved in SP_B (pulmonary surfactant protein B) and StAsp-PSI could explain the cytotoxic activity exerted by StAsp-PSI and StAPs against Gram-positive bacteria. These results and data previously reported suggest that the presence of the PSI domain in mature StAPs could be related to their antimicrobial activity | - |
| dc.format | application/pdf | - |
| dc.format | 7 p. | - |
| dc.language | eng | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | - |
| dc.source | reponame:CIC Digital (CICBA) | - |
| dc.source | instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires | - |
| dc.source | instacron:CICBA | - |
| dc.source.uri | http://digital.cic.gba.gob.ar/handle/11746/5367 | - |
| dc.source.uri | Recurso Completo | - |
| dc.subject | Biología Celular, Microbiología | - |
| dc.title | Swaposin domain of potato aspartic protease (StAsp-PSI) exerts antimicrobial activity on plant and human pathogens | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/submittedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | Comisión de Investigaciones Científicas de la Prov. de Buenos Aires | |
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