Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Petrigh, Romina Sandra | - |
| dc.creator | Fugassa, Martín Horacio | - |
| dc.date | 2016-01-04T20:21:42Z | - |
| dc.date | 2016-01-04T20:21:42Z | - |
| dc.date | 2013-12-06 | - |
| dc.date | 2016-03-30 10:35:44.97925-03 | - |
| dc.date.accessioned | 2019-04-29T15:57:05Z | - |
| dc.date.available | 2019-04-29T15:57:05Z | - |
| dc.identifier | Petrigh, Romina Sandra; Fugassa, Martín Horacio; DNA extraction and a cost-effective detection method for Echinococcus granulosus protoscoleces; Elsevier; Veterinary Parasitology; 198; 3-4; 6-12-2013; 410-413 | - |
| dc.identifier | 0304-4017 | - |
| dc.identifier | http://hdl.handle.net/11336/3324 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/306059 | - |
| dc.description | Most methods of DNA purification from protoscoleces of Echinococcus granulosus involve the use of expensive kits and may also require a second step after extraction for an effective purification. The present work describes an optimized cost-effective method that is fast and simple. This method is based on a chemical lysis with proteinase K with a subsequent one-step PCR detection. In this study we used already available primers and newly designed primers to amplify two fragments of different size corresponding to the mitochondrial cytochrome C oxidase subunit 1 gene. By one-step PCR, both fragments were successfully amplified from even a single protoscolex. This result demonstrates that this method of extraction is efficient even with small amounts of sample and that PCR is highly sensitive. The major advantage of this lysis-PCR method is that it avoids a second step of purification resulting in a simpler and more economical method. Our research will serve as a base for future studies on E. granulosus genotyping, mainly with wild mammals with a low number of cysts. | - |
| dc.description | Fil: Petrigh, Romina Sandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata; Argentina | - |
| dc.description | Fil: Fugassa, Martín Horacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401713005049 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2013.09.010 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | SINGLE PROTOSCOLEX LYSIS | - |
| dc.subject | ONE-STEP PCR | - |
| dc.subject | ECHINOCOCCUS GENOTYPING | - |
| dc.subject | Ciencias Veterinarias | - |
| dc.subject | Ciencias Veterinarias | - |
| dc.subject | CIENCIAS AGRÍCOLAS | - |
| dc.title | DNA extraction and a cost-effective detection method for Echinococcus granulosus protoscoleces | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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