Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Pizarro, María Dolores | - |
| dc.creator | Mediavilla, Maria Gabriela | - |
| dc.creator | Berardi, Florencia | - |
| dc.creator | Tiribelli, Claudio | - |
| dc.creator | Rodriguez, Joaquin Valentin | - |
| dc.creator | Mamprin, María Eugenia | - |
| dc.date | 2016-12-05T19:53:13Z | - |
| dc.date | 2016-12-05T19:53:13Z | - |
| dc.date | 2014-03 | - |
| dc.date | 2016-11-24T19:37:31Z | - |
| dc.date.accessioned | 2019-04-29T15:52:36Z | - |
| dc.date.available | 2019-04-29T15:52:36Z | - |
| dc.date.issued | 2014-03 | - |
| dc.identifier | Pizarro, María Dolores; Mediavilla, Maria Gabriela; Berardi, Florencia; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation; Mexican Assoc Hepatology; Annals Of Hepatology; 13; 2; 3-2014; 256-264 | - |
| dc.identifier | 1665-2681 | - |
| dc.identifier | http://hdl.handle.net/11336/8812 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/304369 | - |
| dc.description | Introduction: This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcar-bamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods: After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results: At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion: We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0oC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system. | - |
| dc.description | Fil: Pizarro, María Dolores. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliânica y Aplicada; Argentina | - |
| dc.description | Fil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliânica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Berardi, Florencia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliânica y Aplicada; Argentina | - |
| dc.description | Fil: Tiribelli, Claudio. Universidad de Trieste. Centro Studi Fegato; Italia | - |
| dc.description | Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (argentina- Italia) de Investigaciones En Criobiologia Cliânica y Aplicada; Argentina | - |
| dc.description | Fil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Cs.fisiologicas. Area Farmacologia; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Mexican Assoc Hepatology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.annalsofhepatology.com/vista?accion=viewArticle&idart=984 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | AMMONIA METABOLISM | - |
| dc.subject | LIVER MICROORGANS | - |
| dc.subject | COLD STORAGE | - |
| dc.subject | BG35 | - |
| dc.subject | PRESERVATION SOLUTIONS | - |
| dc.subject | LIVER PRESERVATION | - |
| dc.subject | Otras Medicina Básica | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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