Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Britos, Claudia Noelia | - |
| dc.creator | Lapponi, María José | - |
| dc.creator | Cappa, Valeria Alejandra | - |
| dc.creator | Rivero, Cintia Wanda | - |
| dc.creator | Trelles, Jorge Abel | - |
| dc.date | 2018-06-01T15:58:15Z | - |
| dc.date | 2018-06-01T15:58:15Z | - |
| dc.date | 2016-06 | - |
| dc.date | 2018-05-31T15:11:22Z | - |
| dc.date.accessioned | 2019-04-29T15:50:29Z | - |
| dc.date.available | 2019-04-29T15:50:29Z | - |
| dc.date.issued | 2016-06 | - |
| dc.identifier | Britos, Claudia Noelia; Lapponi, María José; Cappa, Valeria Alejandra; Rivero, Cintia Wanda; Trelles, Jorge Abel; Biotransformation of halogenated nucleosides by immobilized Lactobacillus animalis 2′- N -deoxyribosyltransferase; Elsevier Science Sa; Journal of Fluorine Chemistry; 186; 6-2016; 91-96 | - |
| dc.identifier | 0022-1139 | - |
| dc.identifier | http://hdl.handle.net/11336/46978 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/303444 | - |
| dc.description | An immobilized biocatalyst with 2′-N-deoxyribosyltransferase (NDT) activity, Lactobacillus animalis NDT (LaNDT), was developed from cell free extracts. LaNDT was purified, characterized and then immobilized by ionic interaction. Different process parameters were optimized, resulting in an active derivative (2.6 U/g) able to obtain 1.75 mg/g of 5-fluorouracil-2′-deoxyriboside, an antimetabolite known as floxuridine, used in gastrointestinal cancer treatment. Furthermore, immobilized LaNDT was satisfactorily used to obtain at short reaction times other halogenated pyrimidine and purine 2′-deoxynucleosides such as 6-chloropurine-2′-deoxyriboside (4.9 U/g), 6-bromopurine-2′-deoxyriboside (4.3 U/g), 6-chloro-2-fluoropurine-2′-deoxyriboside (5.4 U/g), 5-bromo-2′-deoxyuridine (2.8 U/g) and 5-chloro-2′-deoxyuridine (1.8 U/g) compounds of pharmaceutical interest in antiviral or antitumor treatments. Besides, increasing the biocatalyst amount 8 times per volume unit allowed obtaining a 5-fold improvement in floxuridine biotransformation. The developed biocatalyst proved to be effective for the biosynthesis of a wide spectrum of nucleoside analogues by employing an economical, simple and environmentally friendly methodology. | - |
| dc.description | Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina | - |
| dc.description | Fil: Lapponi, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina | - |
| dc.description | Fil: Cappa, Valeria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina | - |
| dc.description | Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina | - |
| dc.description | Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science Sa | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.jfluchem.2016.04.012 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022113916301002 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Green chemistry | - |
| dc.subject | Floxuridine | - |
| dc.subject | DEAE-Sepharose | - |
| dc.subject | Antiviral agents | - |
| dc.subject | Antitumor compounds | - |
| dc.subject | Enzyme stabilization | - |
| dc.subject | Ingeniería Química | - |
| dc.subject | Ingeniería Química | - |
| dc.subject | INGENIERÍAS Y TECNOLOGÍAS | - |
| dc.title | Biotransformation of halogenated nucleosides by immobilized Lactobacillus animalis 2′- N -deoxyribosyltransferase | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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