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dc.creatorLivore, Veronica Ines-
dc.creatorUttaro, Antonio Domingo-
dc.date2018-03-06T18:12:44Z-
dc.date2018-03-06T18:12:44Z-
dc.date2015-01-
dc.date2018-03-02T14:22:22Z-
dc.date.accessioned2019-04-29T15:48:20Z-
dc.date.available2019-04-29T15:48:20Z-
dc.date.issued2015-01-
dc.identifierLivore, Veronica Ines; Uttaro, Antonio Domingo; Biosynthesis of very long chain fatty acids in Trypanosoma cruzi; Springer; Parasitology Research; 114; 1; 1-2015; 265-271-
dc.identifier0932-0113-
dc.identifierhttp://hdl.handle.net/11336/37971-
dc.identifier1432-1955-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302505-
dc.descriptionTrypanosoma brucei and Trypanosoma cruzi showed similar fatty acid (FA) compositions, having a high proportion of unsaturated FAs, mainly 18:2Δ9,12 (23–39 %) and 18:1Δ9 (11–17 %). C22 polyunsaturated FAs are in significant amounts only in T. brucei (12–20 %) but represent a mere 2 % of total FAs in T. cruzi. Both species have also similar profiles of medium- and long-chain saturated FAs, from 14:0 to 20:0. Interestingly, procyclic and bloodstream forms of T. brucei lack very long chain FAs (VLCFAs), whereas epimastigotes and trypomastigotes of T. cruzi contain 22:0 (0.1–0.2 %), 24:0 (1.5–2 %), and 26:0 (0.1–0.2 %). This is in agreement with the presence of an additional FA elongase gene (TcELO4) in T. cruzi. TcELO4 was expressed in a Saccharomyces cerevisiae mutant lacking the endogenous ScELO3, rescuing the synthesis of saturated and hydroxylated C26 FAs in the yeast. Expression of TcELO4 also rescued the synthetic lethality of a ScELO2, ScELO3 double mutation, and the VLCFA profile of the transformed yeast was similar to that found in T. cruzi. By identifying TcELO4 as the enzyme responsible for the elongation of FA from 16:0 and 18:0 up to 26:0, with 24:0 being the preferred product, this work completed the characterization of FA elongases in Trypanosoma spp.-
dc.descriptionFil: Livore, Veronica Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina-
dc.descriptionFil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSpringer-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00436-014-4188-y-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00436-014-4188-y-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/7230-
dc.subjectELONGASE-
dc.subjectLIPIDS-
dc.subjectTRYPANOSOMA CRUZI-
dc.subjectVERY LONG CHAIN FATTY ACID-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleBiosynthesis of very long chain fatty acids in Trypanosoma cruzi-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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