Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Ruzal, Sandra Monica | - |
| dc.creator | Bustos, Patricia Laura | - |
| dc.creator | Sanchez, Carmen | - |
| dc.date | 2017-07-18T18:53:59Z | - |
| dc.date | 2017-07-18T18:53:59Z | - |
| dc.date | 2013-02 | - |
| dc.date | 2017-07-18T15:35:55Z | - |
| dc.date.accessioned | 2019-04-29T15:47:34Z | - |
| dc.date.available | 2019-04-29T15:47:34Z | - |
| dc.date.issued | 2013-02 | - |
| dc.identifier | Ruzal, Sandra Monica; Bustos, Patricia Laura; Sanchez, Carmen; “A La Recherche” of Functions for the Spore Protein SASP-E from Bacillus subtilis; Korean Society Microbiology; Journal of Microbiology and Biotechnology; 23; 1; 2-2013; 15-21 | - |
| dc.identifier | 1017-7825 | - |
| dc.identifier | http://hdl.handle.net/11336/20834 | - |
| dc.identifier | 1738-8872 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/302138 | - |
| dc.description | We previously observed that Bacillus subtilis spores from sspE mutants presented a lower germination capacity in media containing high salt concentrations (0.9 M NaCl). This deficiency was attributed to the absence of SASP-E (gamma-type small-acid-soluble protein), rich in osmocompatible amino acids released by degradation. Herein we observed that, in addition, this mutant spore presented a reduced capacity to use L-alanine as germinant (L-ala pathway), required longer times to germinate in calcium dipicolinate (Ca2+-DPA), but germinated well in asparagine, glucose, fructose, and potassium chloride (AGFK pathway). Moreover, mild sonic treatment of mutant spores partially recovered their germination capacity in L-ala. Spore qualities were also altered, since sporulating colonies from the sspE mutant showed a pale brownish color, a higher adherence to agar plates, and lower autofluorescence, properties related to their spore coat content. Furthermore, biochemical analysis showed a reduced partition in hexadecane and a higher content of Ca2+-DPA when compared with its isogenic wild-type control. Coat protein preparations showed a different electrophoretic pattern, in particular when detected with antibodies against CotG and CotE. The complementation with a wild-type sspE gene in a plasmid allowed for recovering the wild-type coat phenotype. This is the first report of a direct involvement of SASP-E in the spore coat assembly during the differentiation program of sporulation. | - |
| dc.description | Fil: Ruzal, Sandra Monica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Bustos, Patricia Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Sanchez, Carmen. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Korean Society Microbiology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4014/jmb.1206.06028 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.jmb.or.kr/journal/viewJournal.html?year=2013&vol=23&num=1&page=15 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/20834 | - |
| dc.subject | Bacillus subtilis | - |
| dc.subject | Spores | - |
| dc.subject | SASP-E | - |
| dc.subject | Coat | - |
| dc.subject | Biología Celular, Microbiología | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | “A La Recherche” of Functions for the Spore Protein SASP-E from Bacillus subtilis | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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