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dc.creatorGarcia Manzano, Maria Florencia-
dc.creatorAlvarez Igarzabal, Cecilia Ines-
dc.date2017-02-14T21:03:15Z-
dc.date2017-02-14T21:03:15Z-
dc.date2011-04-
dc.date2017-02-14T14:35:39Z-
dc.date.accessioned2019-04-29T15:43:07Z-
dc.date.available2019-04-29T15:43:07Z-
dc.date.issued2011-04-
dc.identifierGarcia Manzano, Maria Florencia; Alvarez Igarzabal, Cecilia Ines; Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction; Elsevier Science; Journal Of Molecular Catalysis B: Enzymatic; 72; 1-2; 4-2011; 28-35-
dc.identifier1381-1177-
dc.identifierhttp://hdl.handle.net/11336/13020-
dc.identifier1873-3158-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300259-
dc.descriptionNovel hydrogels based on N-acryloyl-tris(hydroxymethyl) aminomethane (NAT), 2-aminoethyl methacrylate (AEMA) and N,N-methylenebisacrylamide (BIS) were prepared and applied for immobilization of lipase. Two procedures were previously performed on the hydrogels as follows: (a) modification with epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) or (b) grafting with AEMA, using ceric ammonium sulfate as an initiator, and subsequent reaction with ECH. In the hydrolysis reactions, high percentages of lipase activity were reached with products whose enzyme immobilization were performed on both BDGE-containing products and grafted matrices, since the enzyme was bound spaced from the matrices and more likely to interact with the substrate. The best derivatives preserved 86.7?89.5% of the activity corresponding to the soluble enzyme.-
dc.descriptionFil: Garcia Manzano, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina-
dc.descriptionFil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138111771100124X-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.molcatb.2011.04.020-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectHYDROGEL-
dc.subjectMODIFICATION-
dc.subjectOMMOBILIZATION-
dc.subjectCANDIDA RUGOSA LIPASE-
dc.subjectQuímica Orgánica-
dc.subjectCiencias Químicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleImmobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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