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dc.creatorBublik, Débora Rosa-
dc.creatorScolz, Massimiliano-
dc.creatorTriolo, Gianluca-
dc.creatorMonte, Martin-
dc.creatorSchneider, Claudio-
dc.date2018-12-18T18:06:06Z-
dc.date2018-12-18T18:06:06Z-
dc.date2010-02-
dc.date2018-11-22T15:28:41Z-
dc.date.accessioned2019-04-29T15:43:04Z-
dc.date.available2019-04-29T15:43:04Z-
dc.date.issued2010-02-
dc.identifierBublik, Débora Rosa; Scolz, Massimiliano; Triolo, Gianluca; Monte, Martin; Schneider, Claudio; Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 285; 8; 2-2010; 5274-5281-
dc.identifier0021-9258-
dc.identifierhttp://hdl.handle.net/11336/66674-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300246-
dc.descriptionp21CIP1/WAF1 belongs to the CIP/KIP family of Cdk inhibitors, and its expression is tightly controlled during the cell cycle, mainly by transcriptional and post-translational mechanisms. Fine regulation of p21 CIP1/WAF1 levels is critical for cell cycle control and for cellular response to stress. In the present work, we describe a novel mechanism to modulate p21CIP1/WAF1 levels mediated by the human GTSE-1 (G 2 and S phase-expressed-1) protein. Our results provide evidence that hGTSE-1 protects p21CIP1/WAF1 from proteasome-dependent degradation as part of a functional complex containing the Hsp90-bindingTPR protein WISp39. We further show that the hGTSE-1 N-terminal portion is sufficient for p21 CIP1/WAF1 binding and stabilization. Finally, we demonstrate that hGTSE-1 mediated-p21CIP1/WAF1 stabilization is clearly involved in the ability of cells to counter-act cytotoxicity induced by the microtubule poison paclitaxel. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.-
dc.descriptionFil: Bublik, Débora Rosa. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia-
dc.descriptionFil: Scolz, Massimiliano. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia-
dc.descriptionFil: Triolo, Gianluca. Genome Stability Laboratory; Italia-
dc.descriptionFil: Monte, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia-
dc.descriptionFil: Schneider, Claudio. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia. Università di Udine; Italia-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1074/jbc.M109.045948-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/285/8/5274-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectGTSE-
dc.subjectp21-
dc.subjecttaxol-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleHuman GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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