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dc.creatorVela Gurovic, Maria Soledad-
dc.creatorDíaz Lanza, A. María-
dc.creatorBoyano Adánez, María Del Carmen-
dc.creatorEstañ Omaña, M. Cristina-
dc.creatorGañán Gõmez, Irene-
dc.creatorMurray, Ana Paula-
dc.creatorSancho Lõpez, Pilar-
dc.date2018-11-12T15:02:11Z-
dc.date2018-11-12T15:02:11Z-
dc.date2011-04-
dc.date2018-11-05T19:06:03Z-
dc.date.accessioned2019-04-29T15:42:13Z-
dc.date.available2019-04-29T15:42:13Z-
dc.date.issued2011-04-
dc.identifierVela Gurovic, Maria Soledad; Díaz Lanza, A. María; Boyano Adánez, María Del Carmen; Estañ Omaña, M. Cristina; Gañán Gõmez, Irene; et al.; Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines; John Wiley & Sons Ltd; Phytotherapy Research; 25; 4; 4-2011; 603-610-
dc.identifier0951-418X-
dc.identifierhttp://hdl.handle.net/11336/64229-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299959-
dc.descriptionNatural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC 50 values of 1.98 ± 0.12 μm and 3.52 ± 0.14 μm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (Δψm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of Δψm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment. Copyright © 2010 John Wiley & Sons, Ltd.-
dc.descriptionFil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad de Alcalá; España-
dc.descriptionFil: Díaz Lanza, A. María. Universidad de Alcalá; España-
dc.descriptionFil: Boyano Adánez, María Del Carmen. Universidad de Alcalá; España-
dc.descriptionFil: Estañ Omaña, M. Cristina. Universidad de Alcalá; España-
dc.descriptionFil: Gañán Gõmez, Irene. Universidad de Alcalá; España-
dc.descriptionFil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina-
dc.descriptionFil: Sancho Lõpez, Pilar. Universidad de Alcalá; España-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherJohn Wiley & Sons Ltd-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1002/ptr.3310-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.3310-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/16932-
dc.subjectAPOPTOSIS-
dc.subjectDEQUALINIUM-
dc.subjectHELIANTRIOL B2-
dc.subjectK562 CELL LINE-
dc.subjectLEUKEMIA-
dc.subjectNB4 CELL LINE-
dc.subjectOtras Ciencias Químicas-
dc.subjectCiencias Químicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleCytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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