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dc.provenanceCONICET-
dc.creatorDiaz Gomez, Maria Isabel-
dc.creatorFanelli, Silvia Laura-
dc.creatorDelgado, Aurora Maria-
dc.creatorBietto, Florencia Matilde-
dc.creatorCastro, Jose Alberto-
dc.creatorCastro, Gerardo Daniel-
dc.date2017-10-05T18:08:51Z-
dc.date2017-10-05T18:08:51Z-
dc.date2008-11-
dc.date2017-09-26T18:59:46Z-
dc.date.accessioned2019-04-29T15:39:11Z-
dc.date.available2019-04-29T15:39:11Z-
dc.date.issued2008-11-
dc.identifierDiaz Gomez, Maria Isabel; Fanelli, Silvia Laura; Delgado, Aurora Maria; Bietto, Florencia Matilde; Castro, Jose Alberto; et al.; Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet; Sage Publications Ltd; Toxicology And Industrial Health; 24; 10; 11-2008; 625-634-
dc.identifier0748-2337-
dc.identifierhttp://hdl.handle.net/11336/25990-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298611-
dc.descriptionHighly purified rat-liver nuclei were previously shown to have nuclear ethanol (EtOH) metabolizing system able to bioactivate alcohol to acetaldehyde and 1-hydroxyethyl radicals. These reactive metabolites were able to covalently bind to nuclear proteins and lipids potentially being able to provoke oxidative stress of nuclear components. In this study, the above-mentioned possibility was explored. Sprague Dawley male rats (125–150 g) were fed a standard Lieber and De Carli liquid diet for 28 days. Controls were pair-fed with a diet, in which EtOH was isocalorically replaced with carbohydrate. The presence of a chlorzoxazone hydroxylase activity inducible by the repetitive EtOH drinking further suggested the presence of CYP2E1 in the highly purified nuclei. Nuclei from EtOH-drinking rats evidenced significantly increased susceptibility to a t-butyl hydroperoxide challenge as detected by chemiluminescence emission, increased formation of protein carbonyls, and decreased content of protein sulfhydryls. In contrast, no significant changes in the nuclear lipid hydroperoxides formation or even decreases in the 8-oxo-7,8-dihydro-2-deoxyguanosine were observed. No significant differences were observed in different parameters of the alkaline Comet assay. In immunohistochemical studies performed, no expression of p53 was observed in the livers of the animals under the experimental conditions tested. Since nuclear proteins and lipids are known to play a role in cell growth, differentiation, repair and signaling, their alterations by either oxidative stress, or by covalent binding might be of relevance to liver tumor promotion.-
dc.descriptionFil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina-
dc.descriptionFil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina-
dc.descriptionFil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina-
dc.descriptionFil: Bietto, Florencia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina-
dc.descriptionFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina-
dc.descriptionFil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
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dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSage Publications Ltd-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0748233708101207-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/0748233708101207-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/25990-
dc.subjectALCOHOL-
dc.subjectCYP2E1-
dc.subjectETHANOL-
dc.subjectNUCLEI-
dc.subjectMICROSOMES-
dc.subjectBioquímica y Biología Molecular-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleDeleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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