Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Regiart, Daniel Matias Gaston | - |
| dc.creator | Fernández Baldo, Martín Alejandro | - |
| dc.creator | Spotorno, Viviana G. | - |
| dc.creator | Bertolino, Franco Adrián | - |
| dc.creator | Raba, Julio | - |
| dc.date | 2016-05-20T20:26:16Z | - |
| dc.date | 2016-05-20T20:26:16Z | - |
| dc.date | 2013-03 | - |
| dc.date | 2016-05-16T20:17:34Z | - |
| dc.date.accessioned | 2019-04-29T15:35:38Z | - |
| dc.date.available | 2019-04-29T15:35:38Z | - |
| dc.date.issued | 2016-05-20T20:26:16Z | - |
| dc.date.issued | 2016-05-20T20:26:16Z | - |
| dc.date.issued | 2013-03 | - |
| dc.date.issued | 2016-05-16T20:17:34Z | - |
| dc.identifier | Regiart, Daniel Matias Gaston; Fernández Baldo, Martín Alejandro; Spotorno, Viviana G.; Bertolino, Franco Adrián; Raba, Julio; Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform; Elsevier; Biosensors & Bioelectronics; 41; 3-2013; 211-217 | - |
| dc.identifier | 0956-5663 | - |
| dc.identifier | http://hdl.handle.net/11336/5756 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297377 | - |
| dc.description | In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique. | - |
| dc.description | Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina | - |
| dc.description | Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina | - |
| dc.description | Fil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina | - |
| dc.description | Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina | - |
| dc.description | Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566312005441 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bios.2012.08.020 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2012.08.020 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Microfluidic immunosensor | - |
| dc.subject | Clenbuterol | - |
| dc.subject | Bovine hair samples | - |
| dc.subject | Gold nanoparticles | - |
| dc.subject | Magnetic micro particles | - |
| dc.subject | Química Analítica | - |
| dc.subject | Ciencias Químicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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