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dc.creatorSosa, Liliana del Valle-
dc.creatorGutiérrez, Silvina-
dc.creatorPetiti, Juan Pablo-
dc.creatorVaca, Alicia Maldré del Valle-
dc.creatorde Paul, Ana Lucia-
dc.creatorTorres, Alicia Ines-
dc.date2017-01-18T20:55:22Z-
dc.date2017-01-18T20:55:22Z-
dc.date2013-04-
dc.date2017-01-13T19:47:41Z-
dc.date.accessioned2019-04-29T15:35:27Z-
dc.date.available2019-04-29T15:35:27Z-
dc.date.issued2017-01-18T20:55:22Z-
dc.date.issued2017-01-18T20:55:22Z-
dc.date.issued2013-04-
dc.date.issued2017-01-13T19:47:41Z-
dc.identifierSosa, Liliana del Valle; Gutiérrez, Silvina; Petiti, Juan Pablo; Vaca, Alicia Maldré del Valle; de Paul, Ana Lucia; et al.; Cooperative effect of E2 and FGF2 on lactotroph proliferation triggered by signaling initiated at the plasma membrane; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 305; 1; 4-2013; 45-49-
dc.identifier0193-1849-
dc.identifierhttp://hdl.handle.net/11336/11602-
dc.identifier1522-1555-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297319-
dc.descriptionIn the present work, we investigated the effect of 17β-estradiol (E2) and basic fibroblast growth factor 2 (FGF2) on the lactotroph cell-proliferative response and the related membrane-initiated signaling pathway. Anterior pituitary mixed-cell cultures of random, cycling 3-mo-old female rats were treated with 10 nM E2, E2 membrane-impermeable conjugated BSA (E2-BSA), PPT (ERα agonist), and DPN (ERβ agonist) alone or combined with FGF2 (10 ng/ml) for 30 min or 4 h. Although our results showed that the uptake of BrdU into the nucleus of lactotrophs was not modified by E2 or FGF2 alone, a significant increase in the lactotroph uptake of BrdU was observed after E2/FGF2 coincubation, with this effect being mimicked by PPT/FGF2. These proliferative effects were blocked by ICI 182,780 or PD-98059. The involvement of membrane ER in the proliferative response of prolactin cells induced by the steroid and FGF2 coincubation was confirmed using E2-BSA, and the association between ERα and FGF receptor was observed after E2/FGF2 treatment by immunoprecipitation. A significant increase in the ERK1/2 expression was noted after E2, E2-BSA, PPT, and FGF2 alone, which was more noticeable after E2-BSA/FGF2, E2/FGF2, or PPT/FGF2 treatments. This study provides evidence that E2 and FGF2 exert a cooperative effect on the lactotroph proliferation principally by signaling initiated at the plasma membrane triggering a genomic effect mediated by MEK/ERK1/2, a common signaling pathway, that finally regulates the lactotroph population, thus contributing to pituitary plasticity.-
dc.descriptionFil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina-
dc.descriptionFil: Gutiérrez, Silvina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina-
dc.descriptionFil: Petiti, Juan Pablo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina-
dc.descriptionFil: Vaca, Alicia Maldré del Valle. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina-
dc.descriptionFil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina-
dc.descriptionFil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Centro de Microscopia Electronica; Argentina-
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dc.languageeng-
dc.publisherAmerican Physiological Society-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1152/ajpendo.00027.2013-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://ajpendo.physiology.org/content/305/1/E41-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subject17beta-estradiol-
dc.subjectMembrane RE-
dc.subjectFGF2-
dc.subjectLactotroph cells-
dc.subjectOtras Ciencias de la Salud-
dc.subjectCiencias de la Salud-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleCooperative effect of E2 and FGF2 on lactotroph proliferation triggered by signaling initiated at the plasma membrane-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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