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dc.provenanceCONICET-
dc.creatorReischer, Georg H.-
dc.creatorEbdon, James E.-
dc.creatorBauer, Johanna M.-
dc.creatorSchuster, Nathalie-
dc.creatorAhmed, Warish-
dc.creatorÅström, Johan-
dc.creatorBlanch, Anicet R.-
dc.creatorBlöschl, Günter-
dc.creatorByamukama, Denis-
dc.creatorCoakley, Tricia-
dc.creatorFerguson, Christobel-
dc.creatorGoshu, Goraw-
dc.creatorKo, GwangPyo-
dc.creatorde Roda Husman, Ana Maria-
dc.creatorMushi, Douglas-
dc.creatorPoma, Hugo Ramiro-
dc.creatorPradhan, Bandana-
dc.creatorRajal, Verónica Beatriz-
dc.creatorSchade, Margit A.-
dc.creatorSommer, Regina-
dc.creatorTaylor, Huw-
dc.creatorToth, Erika M.-
dc.creatorVrajmasu, Virgil-
dc.creatorWuertz, Stefan-
dc.creatorMach, Robert L.-
dc.creatorFarnleitner, Andreas H.-
dc.date2016-03-10T20:06:02Z-
dc.date2016-03-10T20:06:02Z-
dc.date2013-06-
dc.date2016-03-30 10:35:44.97925-03-
dc.date.accessioned2019-04-29T15:33:37Z-
dc.date.available2019-04-29T15:33:37Z-
dc.date.issued2013-06-
dc.identifierReischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; et al.; Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents; American Chemical Society; Environmental Science & Technology; 47; 15; 6-2013; 8548-8556-
dc.identifier0013-936X-
dc.identifierhttp://hdl.handle.net/11336/4720-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296543-
dc.descriptionNumerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.-
dc.descriptionFil: Reischer, Georg H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria-
dc.descriptionFil: Ebdon, James E.. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido-
dc.descriptionFil: Bauer, Johanna M.. Vienna University of Technology; Austria-
dc.descriptionFil: Schuster, Nathalie. Vienna University of Technology; Austria-
dc.descriptionFil: Ahmed, Warish. CSIRO Land and Water; Australia-
dc.descriptionFil: Åström, Johan. Chalmers University Of Technology; Suecia-
dc.descriptionFil: Blanch, Anicet R.. Universidad de Barcelona. Departamento de Bioquimica; España-
dc.descriptionFil: Blöschl, Günter. Vienna University of Technology; Austria-
dc.descriptionFil: Byamukama, Denis. Makerere University. Department of Biochemistry; Uganda-
dc.descriptionFil: Coakley, Tricia. University Of Kentucky; Estados Unidos-
dc.descriptionFil: Ferguson, Christobel. ALS Water Sciences Group; Australia-
dc.descriptionFil: Goshu, Goraw. Bahir Dar University. Blue Nile Water Institute. College of Agriculture and Environmental Sciences; Etiopía-
dc.descriptionFil: Ko, GwangPyo. Seoul National University; Corea del Sur-
dc.descriptionFil: de Roda Husman, Ana Maria. National Institute for Public Health and the Environment ; Países Bajos-
dc.descriptionFil: Mushi, Douglas. Sokoine University; Tanzania-
dc.descriptionFil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina-
dc.descriptionFil: Pradhan, Bandana. Tribhuvan University; Nepal-
dc.descriptionFil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina-
dc.descriptionFil: Schade, Margit A.. Bavarian Environment Agency; Alemania-
dc.descriptionFil: Sommer, Regina. InterUniversity Cooperation Centre Water & Health; Austria. Medical University of Vienna. Institute for Hygiene and Applied Immunology, Water Hygiene; Austria-
dc.descriptionFil: Taylor, Huw. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido-
dc.descriptionFil: Toth, Erika M.. Eötvös Lorand University. Department of Microbiology. Biological Institute; Hungría-
dc.descriptionFil: Vrajmasu, Virgil. Veterinary State Laboratory; Rumania-
dc.descriptionFil: Wuertz, Stefan. University Of California At Davis; Estados Unidos-
dc.descriptionFil: Mach, Robert L.. Vienna University of Technology; Austria-
dc.descriptionFil: Farnleitner, Andreas H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherAmerican Chemical Society-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/es304367t-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021%2Fes304367t-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737603/-
dc.relationinfo:eu-repo/semantics/altIdentifier/issn/0013-936X-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/4720-
dc.subjectMicrobial source tracking-
dc.subjectPollution microbiology-
dc.subjectFecal pollution-
dc.subjectBacteroidetes-
dc.subjectQuantitative real-time PCR-
dc.subjectGenetic markers-
dc.subjectWater quality-
dc.subjectBiotecnología Medioambiental-
dc.subjectBiotecnología del Medio Ambiente-
dc.subjectINGENIERÍAS Y TECNOLOGÍAS-
dc.titlePerformance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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