Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Mazzaferro, Laura | - |
| dc.creator | Piñuel, Maria Lucrecia | - |
| dc.creator | Erra Balsells, Rosa | - |
| dc.creator | Giudicessi, Silvana Laura | - |
| dc.creator | Breccia, Javier Dario | - |
| dc.date | 2017-03-20T20:37:17Z | - |
| dc.date | 2017-03-20T20:37:17Z | - |
| dc.date | 2012-01 | - |
| dc.date | 2017-03-20T17:40:53Z | - |
| dc.date.accessioned | 2019-04-29T15:33:37Z | - |
| dc.date.available | 2019-04-29T15:33:37Z | - |
| dc.date.issued | 2012-01 | - |
| dc.identifier | Mazzaferro, Laura; Piñuel, Maria Lucrecia; Erra Balsells, Rosa; Giudicessi, Silvana Laura; Breccia, Javier Dario; Transglycosylation specificity of Acremonium sp. α-rhamnosyl-β-glucosidase and its application to the synthesis of the new fluorogenic substrate 4-methylumbelliferyl-rutinoside; Elsevier; Carbohydrate Research; 347; 1; 1-2012; 69-75 | - |
| dc.identifier | 0008-6215 | - |
| dc.identifier | http://hdl.handle.net/11336/14115 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296540 | - |
| dc.description | Transglycosylation potential of the fungal diglycosidase α-rhamnosyl-β-glucosidase was explored. The biocatalyst was shown to have broad acceptor specificity toward aliphatic and aromatic alcohols. This feature allowed the synthesis of the diglycoconjugated fluorogenic substrate 4-methylumbelliferyl-rutinoside. The synthesis was performed in one step from the corresponding aglycone, 4-methylumbelliferone, and hesperidin as rutinose donor. 4-Methylumbelliferyl-rutinoside was produced in an agitated reactor using the immobilized biocatalyst with a 16% yield regarding the sugar acceptor. The compound was purified by solvent extraction and silica gel chromatography. MALDI-TOF/TOF data recorded for the [M+Na]+ ions correlated with the theoretical monoisotopic mass (calcd [M+Na]+: 507.44 m/z; obs. [M+Na]+: 507.465 m/z). 4-Methylumbelliferyl-rutinoside differs from 4-methylumbelliferyl-glucoside in the rhamnosyl substitution at the C-6 of glucose, and this property brings about the possibility to explore in nature the occurrence of endo-β-glucosidases by zymographic analysis. | - |
| dc.description | Fil: Mazzaferro, Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias de la Tierra y Ambientales de la Pampa; Argentina. Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina | - |
| dc.description | Fil: Piñuel, Maria Lucrecia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias de la Tierra y Ambientales de la Pampa; Argentina. Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina | - |
| dc.description | Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina | - |
| dc.description | Fil: Giudicessi, Silvana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones En Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina | - |
| dc.description | Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias de la Tierra y Ambientales de la Pampa; Argentina. Universidad Nacional de la Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Quimica; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.carres.2011.11.008 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0008621511005556 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/14115 | - |
| dc.subject | DIGLYCOSIDASE | - |
| dc.subject | HESPERIDIN | - |
| dc.subject | ZYMOGRAM | - |
| dc.subject | ACREMONIUM SP. DSM 24697 | - |
| dc.subject | Química Orgánica | - |
| dc.subject | Ciencias Químicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Transglycosylation specificity of Acremonium sp. α-rhamnosyl-β-glucosidase and its application to the synthesis of the new fluorogenic substrate 4-methylumbelliferyl-rutinoside | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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