Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Chazarreta Cifré, Lorena Susana | - |
| dc.creator | Alemany, Mariana | - |
| dc.creator | de Mendoza, Diego | - |
| dc.creator | Altabe, Silvia Graciela | - |
| dc.date | 2016-03-18T18:12:41Z | - |
| dc.date | 2016-03-18T18:12:41Z | - |
| dc.date | 2013-02 | - |
| dc.date | 2016-03-30 10:35:44.97925-03 | - |
| dc.date.accessioned | 2019-04-29T15:32:55Z | - |
| dc.date.available | 2019-04-29T15:32:55Z | - |
| dc.date.issued | 2016-03-18T18:12:41Z | - |
| dc.date.issued | 2016-03-18T18:12:41Z | - |
| dc.date.issued | 2013-02 | - |
| dc.date.issued | 2016-03-30 10:35:44.97925-03 | - |
| dc.identifier | Chazarreta Cifré, Lorena Susana; Alemany, Mariana; de Mendoza, Diego; Altabe, Silvia Graciela; Exploring the biosynthesis of unsaturated fatty acids in Bacillus cereus ATCC 14579 and functional characterization of novel acyl-lipid desaturases; American Society for Microbiology; Applied And Environmental Microbiology; 79; 20; 2-2013; 6271-6279 | - |
| dc.identifier | 0099-2240 | - |
| dc.identifier | http://hdl.handle.net/11336/4853 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296272 | - |
| dc.description | At low temperatures, Bacillus cereus synthesizes large amounts of unsaturated fatty acids (UFAs) with double bonds in positions Δ5 and Δ10, as well as Δ5,10 diunsaturated fatty acids. Through sequence homology searches, we identified two open reading frames (ORFs) encoding a putative Δ5 desaturase and a fatty acid acyl-lipid desaturase in the B. cereus ATCC 14579 genome, and these were named BC2983 and BC0400, respectively. Functional characterization of ORFs BC2983 and BC0400 by means of heterologous expression in Bacillus subtilis confirmed that they both encode acyl-lipid desaturases that use phospholipids as the substrates and have Δ5 and Δ10 desaturase activities. Thus, these ORFs were correspondingly named desA (Δ5 desaturase) and desB (Δ10 desaturase). We established that DesA utilizes ferredoxin and flavodoxins (Flds) as electron donors for the desaturation reaction, while DesB preferably employs Flds. In addition, increased amounts of UFAs were found when B. subtilis expressing B. cereus desaturases was subjected to a cold shock treatment, indicating that the activity or the expression of these enzymes is upregulated in response to a decrease in growth temperature. This represents the first work reporting the functional characterization of fatty acid desaturases from B. cereus. | - |
| dc.description | Fil: Chazarreta Cifré, Lorena Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Alemany, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: de Mendoza, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Altabe, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society for Microbiology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/79/20/6271.long | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/pmid/PMC3811188 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128%2FAEM.01761-13 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/10.1128%2FAEM.01761-13 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3811188/ | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/4853 | - |
| dc.subject | BACILLUS CEREUS | - |
| dc.subject | DESASTURASE | - |
| dc.subject | UNSATURATED FATTY ACIDS | - |
| dc.subject | Biología Celular, Microbiología | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Exploring the biosynthesis of unsaturated fatty acids in Bacillus cereus ATCC 14579 and functional characterization of novel acyl-lipid desaturases | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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