Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Pelufo, Valeria | - |
| dc.creator | Lopez Armengol, Maria Fernanda | - |
| dc.creator | Malcotti, Valeria | - |
| dc.creator | Venturino, Andres | - |
| dc.creator | Aisen, Eduardo Gabriel | - |
| dc.date | 2018-03-05T19:15:03Z | - |
| dc.date | 2018-03-05T19:15:03Z | - |
| dc.date | 2015-01 | - |
| dc.date | 2018-03-02T14:19:59Z | - |
| dc.date.accessioned | 2019-04-29T15:32:54Z | - |
| dc.date.available | 2019-04-29T15:32:54Z | - |
| dc.date.issued | 2018-03-05T19:15:03Z | - |
| dc.date.issued | 2018-03-05T19:15:03Z | - |
| dc.date.issued | 2015-01 | - |
| dc.date.issued | 2018-03-02T14:19:59Z | - |
| dc.identifier | Pelufo, Valeria; Lopez Armengol, Maria Fernanda; Malcotti, Valeria; Venturino, Andres; Aisen, Eduardo Gabriel; Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm; Elsevier Science Inc; Theriogenology; 83; 1; 1-2015; 144-151 | - |
| dc.identifier | 0093-691X | - |
| dc.identifier | http://hdl.handle.net/11336/37853 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296266 | - |
| dc.description | Sperm deep freezing procedures for ram semen have considerable variations regarding the steps being employed for cooling, freezing, and addition of cryoprotectants. In this work, we evaluated the effects of the addition of glycerol and/or the disaccharides sucrose and trehalose to hypertonic diluents either before or after cooling from 30°C to 5°C in Merino Australian ram semen cryopreservation. Using optical and transmission electron microscopy techniques, we assessed that glycerol was beneficial to the cooling process independently of its addition at 30°C or 5°C in terms of sperm membrane integrity in different regions of the plasma membrane (acrosomal region, 14.5% higher integrity; postacrosomal region, 8.0% higher integrity [P<0.01]; hypoosmotic swelling test [HOST], 10.8% higher integrity [P<0.001]). Disaccharides were necessary for a better cryopreservation in liquid nitrogen, and the best procedure was their addition after cooling at 5°C (12% higher sperm motility [P<0.001]; 8% higher acrosome integrity, [P<0.05]; 9.5% higher plasma membrane integrity assessed by HOST [P<0.001]). Trehalose showed a greater preservation cryoprotectant capacity than sucrose, as indicated by sperm motility after thawing (8.1% greater [P<0.01]) and by the integrity of the intermediate piece (20% greater [P<0.05]). From these results, we conclude that the best procedure for ram semen cryopreservation in hypertonic disaccharide-containing diluents is the addition of glycerol and trehalose after the cooling process, at 5°C. | - |
| dc.description | Fil: Pelufo, Valeria. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Instituto de Biotecnología Agropecuaria del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Lopez Armengol, Maria Fernanda. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Instituto de Biotecnología Agropecuaria del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Malcotti, Valeria. Gobierno de la Provincia del Neuquén. Ministerio de Desarrollo Territorial; Argentina | - |
| dc.description | Fil: Venturino, Andres. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Instituto de Biotecnología Agropecuaria del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Aisen, Eduardo Gabriel. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias. Instituto de Biotecnología Agropecuaria del Comahue; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science Inc | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.theriogenology.2014.09.007 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X14004968 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/37853 | - |
| dc.subject | COOLING PROCESS | - |
| dc.subject | FROZEN-THAWED RAM SPERMATOZOA | - |
| dc.subject | PLASMA MEMBRANE DOMAINS | - |
| dc.subject | TREHALOSE | - |
| dc.subject | Otras Biotecnología Agropecuaria | - |
| dc.subject | Biotecnología Agropecuaria | - |
| dc.subject | CIENCIAS AGRÍCOLAS | - |
| dc.title | Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.