Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Mejias, Maria Pilar | - |
| dc.creator | Cabrera, Gabriel | - |
| dc.creator | Fernández Brando, Romina Jimena | - |
| dc.creator | Baschkier, Ariela | - |
| dc.creator | Ghersi, Giselle | - |
| dc.creator | Abrey Recalde, Maria Jimena | - |
| dc.creator | Miliwebsky, Elyzabeth | - |
| dc.creator | Meiss, Roberto | - |
| dc.creator | Goldbaum, Fernando Alberto | - |
| dc.creator | Zylberman, Vanesa | - |
| dc.creator | Rivas, Marta | - |
| dc.creator | Palermo, Marina Sandra | - |
| dc.date | 2016-12-13T21:03:11Z | - |
| dc.date | 2016-12-13T21:03:11Z | - |
| dc.date | 2014-04 | - |
| dc.date | 2016-11-25T16:33:54Z | - |
| dc.date.accessioned | 2019-04-29T15:26:30Z | - |
| dc.date.available | 2019-04-29T15:26:30Z | - |
| dc.date.issued | 2014-04 | - |
| dc.identifier | Mejias, Maria Pilar; Cabrera, Gabriel; Fernández Brando, Romina Jimena; Baschkier, Ariela; Ghersi, Giselle; et al.; Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera; American Society For Microbiology; Infection And Immunity; 82; 4; 4-2014; 1491-1499 | - |
| dc.identifier | 0019-9567 | - |
| dc.identifier | http://hdl.handle.net/11336/9311 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/294226 | - |
| dc.description | Hemolytic-uremic syndrome (HUS) is defined as the triad of anemia, thrombocytopenia, and acute kidney injury. Enterohemorrhagic Shiga toxin (Stx)-producing Escherichia coli (EHEC), which causes a prodromal hemorrhagic enteritis, remains the most common etiology of the typical or epidemic form of HUS. Because no licensed vaccine or effective therapy is presently available for human use, we recently developed a novel immunogen based on the B subunit of Shiga toxin 2 (Stx2B) and the enzyme lumazine synthase from Brucella spp. (BLS) (BLS-Stx2B). The aim of this study was to analyze maternal immunization with BLS-Stx2B as a possible approach for transferring anti-Stx2 protection to the offspring. BALB/c female mice were immunized with BLS-Stx2B before mating. Both dams and pups presented comparable titers of anti-Stx2B antibodies in sera and fecal extracts. Moreover, pups were totally protected against a lethal dose of systemic Stx2 injection up to 2 to 3 months postpartum. In addition, pups were resistant to an oral challenge with an Stx2-producing EHEC strain at weaning and did not develop any symptomatology associated with Stx2 toxicity. Fostering experiments demonstrated that anti-Stx2B neutralizing IgG antibodies were transmitted through breast-feeding. Pups that survived the EHEC infection due to maternally transferred immunity prolonged an active and specific immune response that protected them against a subsequent challenge with intravenous Stx2. Our study shows that maternal immunization with BLS-Stx2B was very effective at promoting the transfer of specific antibodies, and suggests that preexposure of adult females to this immunogen could protect their offspring during the early phase of life. | - |
| dc.description | Fil: Mejias, Maria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina | - |
| dc.description | Fil: Cabrera, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina | - |
| dc.description | Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina | - |
| dc.description | Fil: Baschkier, Ariela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina | - |
| dc.description | Fil: Ghersi, Giselle. Inmunova S.a; Argentina | - |
| dc.description | Fil: Abrey Recalde, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina | - |
| dc.description | Fil: Miliwebsky, Elyzabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina | - |
| dc.description | Fil: Meiss, Roberto. Academia Nacional de Medicina. Centro de Estudios Oncológicos. Departamento de Patología; Argentina | - |
| dc.description | Fil: Goldbaum, Fernando Alberto. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Inmunova S.a; Argentina | - |
| dc.description | Fil: Zylberman, Vanesa. Inmunova S.a; Argentina. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina | - |
| dc.description | Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina | - |
| dc.description | Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society For Microbiology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/82/4/1491.long | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.00027-14 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | shiga toxin | - |
| dc.subject | hemolytic uremic syndrome | - |
| dc.subject | vaccine | - |
| dc.subject | protection | - |
| dc.subject | Biología Celular, Microbiología | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.